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Nedic et al., 2021

Briefly, bacterial pellets were lysed in buffer (150 mM NaCl 2 , 50 mM Tris, 10% (v/v) glycerol, 1 mM phenylmethylsulfonyl fluoride (PMSF) at pH 7.4 by bead-beating as described for the ATP assay

A West Point graduate and board-certified orthopedic surgeon, he spent more than 30 years optimizing the performance and physical resilience of Navy SEALs and Special Forces personnel, later completing fellowship training in anti-aging medicine

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