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To obtain additional restraints for structural model determination of the p53 TAD2 -FOXO4 FH complex, we determined paramagnetic relaxation enhancements (PREs) for a set of 7 complexes containing spin-labeled (SL) samples ( 15 N-labeled FOXO4 FH + S46C-SL p53 TAD2 , 15 N-labeled FOXO4 FH + Q52C-SL p53 TAD2 , 15 N-labeled p53 TAD2 + Q112C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + D140C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + N145C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + S159C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + S162C-SL FOXO4 FH )

Growth media was replaced with binding buffer [phenol-free DMEM containing 25 mM HEPES and 0.1% (w/v) BSA] containing 0.7 nM 125 I-Ex(939) or 0.15 nM 125 I-hGLP-1 and increasing concentrations of unlabelled ligand

Parker | Government Finance Adam C

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