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The journey from discovery of the GLP-1 hormone to treatments for diabetes and then obesity was a long and stuttering one, spanning more than four decades and involving hundreds of researchers across academia and the pharmaceutical industry

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The cells were fixed in 4% PFA in PBS for 30 min, permeabilized with 0.2% Triton X-100 in PBS for 5 min, treated with 2 M HCl for 20 min and neutralized with 0.1 M sodium tetraborate for 5 min

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