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For the OCR assays in cells, culture medium was replaced with MAS buffer with 4% BSA, pH 7.2

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We also include additional nutrients to support the glutathione pathway

For example, products marketed online under these names should not be assumed to come from the pharmaceutical developer or to match the clinical-trial material

We therefore measured G protein-dependent cAMP generation and -arrestin-1 and -2 recruitment with exendin-4, exendin-phe1, and exendin-asp3 at two time-points to avoid kinetic artefacts 38 , and calculated the pathway bias by fitting the response data to a modified form of the operational model of agonism 39 (Fig