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In the absence of enough iron in cells, an iron starvation response begins, increasing iron availability by increasing TFCR1 and decreasing the levels of FTH and ferroportin (FPN) ( 2.1.2 Iron homeostasis Under normal circumstances, intracellular iron maintains a subtle balance through the iron transport system ( Iron homeostasis dysregulation is involved in ferroptosis, and multiple iron metabolism regulatory factors work together to maintain iron homeostasis ( 2+ levels and lipid peroxidation, inhibiting ferroptosis ( 2.2 Ferroptosis and lipid metabolism The conversion of lipids into membrane phospholipids and the occurrence of peroxidation are necessary conditions for ferroptosis ( Acyl-CoA synthetase long chain family member 4 (ACSL4) and lysophosphatidylcholine acyltransferase 3 are key enzymes involved in the process of lipid peroxidation (56)

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Lentiviral transduction of mRFP-GFP-LC3 shRNA and autophagic flux determination Lentiviruses encoding short hairpin RNAs (shRNA) to human mRFP-GFP-LC3-puro were constructed by Hanbio Biotechnology (Hanbio, Shanghai, China)