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The GSH level increased over the first 20 generations (two transfers), but subsequently declined and after 60 generations (six transfers) had returned close to that of the control, wild-type strain cultured in the same conditions (Figure S3A)
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In certain cases, this method may remove the need for chromatography altogether and allow for less complex purification methods, such as TFF alone
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Manochkumar J, Doss CGP, El-Seedi HR, Efferth T, Ramamoorthy S (2021) The neuroprotective potential of carotenoids in vitro and in vivo
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