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Lingvay I, Catarig AM, Frias JP, Kumar H, Lausvig NL, le Roux CW, Thielke D, Viljoen A, McCrimmon RJ

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Serotonin is then acetylated by serotonin N-acetyltransferase to form N-acetylserotonin, which is finally methylated by hydroxyindole-O-methyltransferase to produce melatonin (179)

Freezing causes ice crystal formation, which physically disrupts amino acid chains and denatures dissolved peptides