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In vitro barrier models using Caco-2 cells (gut epithelium) or brain endothelial cells could be employed to test the hypothesis that microbiota metabolites, such as butyrateeither administered directly or derived from patient-derived fecal microbiota culturescan strengthen epithelial and endothelial barrier function and reduce oxidative stress [40]

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Compound 102 passed the PAINS (pan assay interference compounds) remover, which filters out compounds that appear as frequent hitters (promiscuous compounds) in many biochemical high throughput screens

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