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Sample type Dilution factor Human serum 1 Mouse serum 3-5 Rat serum 5-8 10% Mouse brain tissue homogenate 1 10% Rat liver tissue homogenate 30-60 HepG2 cell homogenate (5 mgprot/mL) 1 10% Plant tissue homogenate 1-5

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Many drugs can cross the luminal cell membrane but are then metabolized in the gut wall (typically by CYP3A4 e.g