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Finally, the coverslips were incubated for 1 hour in a humidified chamber at RT in secondary Abs (1:200, unconjugated from Jackson ImmunoResearch and conjugated in house as previously described (Coons et al., 1942), donkey anti-mouse IgG: cat# 715-005-151, RRID: AB_2340759

Vitamin B12, or cobalamin, is a natural vitamin found in animal foods like dairy and meat

To prevent or manage vitamin B12 deficiency, incorporating B12-rich foods or supplements into the diet is recommended

According to the THPdb database published in PLOS ONE, the FDA has approved hundreds of peptide and peptide-protein therapeutics to date

Vitamin B12 is primarily sourced from meat, making deficiencies more common among: Vegans and vegetarians Busy and active mums Individuals recovering from surgery Those on weight loss journeys Regular injections throughout the year or a structured course of treatment can help restore and maintain your vitamin levels