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Ex vivo glucose-stimulated insulin secretion from pancreatic islets Culture medium was removed and islet microtissues were equilibrated for 1 h with Krebs Ringer HEPES buffer (131 mM NaCl, 4.8 mM KCl, 1.3 mM CaCl 2 , 25 mM HEPES, 1.2 mM KH 2 PO 4 , 1.2 mM MgSO 4 and 2% BSA) containing 2.8 mM glucose

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