ELISA Kit Components Required Instruments and Reagents Microplate reader (wavelength: 450nm) 37C incubator (CO2 incubator for cell culture is not recommenced.) Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose) Precision single (0.5-10L, 5-50L, 20-200L, 200-1000L) and multi-channel pipette with disposable tips(Calibration is required before use.) Sterile tubes and Eppendorf tubes with disposable tips Absorbent paper and loading slot Deionized or distilled water Assay Procedure Summary Step 1: Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37C
If the patient has a bacterial infection, antibiotics should be added
Type 1 diabetes mellitus (T1D) Pathogenesis of diabetes has not been completely understood yet, but alterations in gut microbial composition have been described in both type 1 (T1D) and type 2 (T2D) diabetes, suggesting a potential role in the disease development [58]
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Protocol design determines frequency patterns from once daily to divided dosing sessions across a single day or structured intervals
