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Nanoparticles were enclosed in dialysis bags (MWCO 1214 kDa, Spectrum Labs, Rancho Dominguez, CA, United States) and immersed in PBS (Phosphate-Buffered Saline, pH 7.4) containing either 0.5 mM or 5 mM ROS

By this point, the cumulative additional fat loss attributable to AOD-9604 (beyond diet and exercise alone) is approximately 2-3 kg in responders

To orient the readers, we synthesis the key disorders covered, highlighting core mechanisms, dosing ranges, therapeutic responses, and evidence strength (Table 1)