The fat redistribution pattern that defines perimenopause, specifically abdominal accumulation, responds to lipolytic GH signaling in a way that caloric restriction alone does not produce
The glycolytic rate was determined using an XF Glycolytic Rate Assay Kit (Agilent, Santa Clara, CA, USA) with final concentrations of 0.5 M rotenone/antimycin A (Rot/AA) and 50 mM 2-deoxy glucose (2-DG) sequentially injected into the SM media
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Therefore, future studies are warranted to ascertain whether Insl5 meets the necessary criteria to be considered a regulator of feeding given that the exogenous studies mentioned above utilize high doses of Insl5, although the initial work is promising