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doi:10.3389/fendo.2023.1085799 12

After cleavage with ULP1 to remove the His-SUMO tag, the eluted proteins were further purified by size-exclusion chromatography using a Superose 6 Increase 10/300 GL column with an elution buffer containing 20 mM HEPES (pH 8.0) and 400 mM NaCl