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Twelve samples preserved in 4% paraformaldehyde undergo ethanol dehydration through graded concentrations (85% for 2 h, 95% for 2 h, followed by 100% for 1 h each) before being cleared in xylene and embedded in paraffin

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McCann MR, Bacher CA, Seguin CA: Exploiting notochord cells for stem cell-based regeneration of the intervertebral disc

Griess assay for nitrite detection Secretion of NO by murine BV-2 cells was quantified indirectly by measuring the accumulation of its stable breakdown product, nitrite [35-38]

Briefly, spectra derived from unlabelled samples were processed through the deconvolution software AMDIS (Stein 1999), using the Fiehn library as database (Kind et al