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Does the 28-day rule apply even if I only used the vial once

The Four Different Types Of Vitamin B12 Supplements: Cyanocobalamin: A supplement and injection of vitamin B12 are the most common forms

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For patients with pernicious anemia, an autoimmune condition that impairs the absorption of Vitamin B12 from the gastrointestinal tract, these injections bypass the need for intrinsic factor, a protein required for Vitamin B12 absorption