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however, this restoration does not consistently correlate with clinical improvement, highlighting the importance of early intervention (Seton et al., 2023)

The RTqPCR mixture consisted of 5 L of SYBR Green Master Mix (Yeasen, Shanghai, China), 1 L of upstream primer, 1 L of downstream primer (with a final concentration of 0.2 M for each primer), and 2 L of cDNA, with enzyme-free water added to a final volume of 10 L

4.5 Gutkidney axis regulation The gutkidney axis refers to the bidirectional interaction between the intestinal microbiota and the kidneys, which plays an increasingly recognized role in CKD progression (43, 44)

2010;25(Suppl 1):S626

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