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Frozen samples of culture medium were thawed on ice, and 2 l of 200 mM TCEP (in 125 mM borate buffer, pH 9.0) was added to 200 l of medium to yield a final concentration of 2 mM TCEP for reducing disulfide bonds

Experimental animals exhibiting NIT were then randomly assigned to either a treatment group (receiving NHPN-1010) or a vehicle treated group (receiving saline), while noise-exposed animals that did not develop NIT were excluded from the study

It is well known that GLP-1 secretion from EECs is regulated by G protein-coupled receptors (GPCRs), but the exact signaling effectors and pathways are yet to be fully revealed

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doi: 10.1007/BF02257619 40