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Tests for statistical significance were conducted by unpaired t -tests using GraphPad software

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Per the NIH Guidelines for the Care and Use of Laboratory animals and strictly adherent to the ARRIVE guidelines (Animal Research: Reporting In Vivo Experiments), animals were kept in a hygienic, naturally ventilated, and pathogen-free environment in conventional stainless-steel cages (6 rats/cage)

(20) Its fairly well understood that Accutane is a known immunosuppressant, as well

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