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Astrocytes treated with berberine can increase the expression of p85 and p-AKT

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The dilution can reduce the matrix difference between sample and standard to get better accuracy

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Neuropathol Appl Neurobiol 28:410417 Chretien F, Le Pavec G, Vallat-Decouvelaere AV, Delisle MB, Uro-Coste E, Ironside JW, Gambetti P, Parchi P, Creminon C, Dormont D, Mikol J, Gray F, Gras G (2004) Expression of excitatory amino acid transporter-1 (EAAT-1) in brain macrophages and microglia of patients with prion diseases