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Besides inhibiting the mitochondrial autophagy process, morphological altered mitochondrial hyperactivated P53 and enhanced SOD enzyme activity ( via mitochondrial cytochrome c release or massive recruitment of CD8 + T cells

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After incubation, 0.5 ml of medium was collected and mixed with 0.5 ml of 1% (w/v) 2-thiobarbituric acid in 50 mM NaOH and 0.5 ml of 2.8% (w/v) trichloroacetic acid

This means that after 24 hours, up to nearly half of the injected vitamin B12 had been absorbed