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10.3389/fphar.2021.739615 21 ChenX.WeiW.LiY.HuangJ.CiX

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Briefly, 1.5 g plasmids DNA and 3 L of 1 mg/ml Polyethylenimine (PEI, 408,727-sigma) transfection reagent were diluted in 250 L of FBS-free DMEM (Invitrogen) separately

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A multicenter trial in Alzheimers disease found no benefit on cognitive or functional outcomes while shifting some biomarkers, a smaller trial in healthy older adults reported improved memory and hippocampal connectivity, and the overall assessment is that it is unlikely to help most healthy adults and that the evidence is not conclusive