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Rats fed olive oil (74% MUFA) showed significantly better glucose tolerance than those fed coconut oil (87% saturated fat), and this benefit was entirely abolished when researchers blocked the GLP-1 receptor - proving the improvement was GLP-1 dependent

Removal of genomic DNA and reverse transcription were carried out with ReverTra Ace qPCR RT Kit (Toyobo, Osaka, Japan) according to the manufacturer's instructions

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Gut microbial -glucuronidases reactivate estrogens as components of the estrobolome that reactivate estrogens