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Real-time RT-PCR Request a detailed protocol Quantitative real-time PCR was performed using a Rotor-Gene 3000 system (Corbett Research) with a QuantiTect SYBR Green RT-PCR kit (Bio-rad) as described (Sun et al., 2011)

In addition, B12 works synergistically with other B vitamins, such as B6 and folate, to support overall cardiovascular health by promoting healthy blood vessel function and reducing inflammation

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however, its activity could be reduced since the increase in PDK1 expression observed in bFLS treated with bTNF- 24 , reducing the flux of glucose carbon to the TCA cycle observed in cells treated with TNF-