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Primary antibodies includes rabbit anti-phospho-H3 (1:100, Cell signaling, cat#9701), rabbit anti-cleaved caspase 3 (1:100, Cell signaling, cat#9661), sheep anti-acetaminophen primary antibody (1:250, Bio-Rad, cat#0016-0104)

For instance, berberine inhibits endothelial ferroptosis and AS by suppressing ACSL4 expression (Jiao et al., 2021)

3 Results 3.1 Recombinant production and purification of carnitine monooxygenase and MSA-DH The catalytic component CntA and the related reductase CntB of carnitine monooxygenase from A

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