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doi: 10.1016/j.focha.2025.100919 Summary Keywords alleviating mechanism, gut microbiota, health benefits, live biotherapeutics, next generation probiotics, probiotics Citation El-Saadony MT, Saad AM, Sitohy M, Alkafaas SS, Dladla M, Ghosh S, Mohammed DM, Ibrahim EH, Fahmy MA, Elkelish A, AbuQamar SF and El-Tarabily KA (2026) Probiotics and human health: biological activities, nutritional aspects, immunomodulatory properties, applications, and future perspectives - a comprehensive review