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10.1016/s0168-1702(99)00121-5 77 ImadaT.NakamuraS.KitamuraN.ShibuyaI.TsubotaK

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Dishes were washed with fresh saline before imaging with an Olympus IX71 microscope with a 60/1.4 NA oil-immersion objective and an OrcaER camera (Hamamatsu), and using MetaFluor software (Molecular Devices)

Diet and exercise must be considered as the first line of treatment, however if they fail to correct metabolic signalling, IGF-1 therapy seems to be promising at metabolic harmonisation