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Biomedicines 10 , 3113 (2022)

f , HEK293T cells were transfected with increasing amounts of mouse Tmeff1 expression plasmid and infected with HSV-1-GFP

One hour later, rats were anesthetized with ketamine (90 mg/kg), xylazine (2.8 mg/kg), and acepromazine (0.72 mg/kg) and transcardially perfused with 0.1 M phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (PFA) in PBS

Bring to a gentle simmer and cook uncovered for 20 minutes, stirring occasionally