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After TMB substrate solution is added, only those wells that contain Rat GROa/CXCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

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Physiol Rev 87:315424 Palmer RM, Ferrige AG, Moncada S (1987) Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor

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