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Preparations of recombinant proteins pGEX-PGRMC1 wt, Y113F or C129S mutant expression vectors were transformed into BL21 (DE3), and the bacteria were incubated in LB with ampicillin at 37 C until OD600 reached at 0.8

FIGURE 4 Effect of TLYY administration on gut microbiota in DSS-induced mice Natural active products can ameliorate ulcerative colitis (UC) through modulation of the gut microbiota

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DSIP (delta sleep-inducing peptide) is a nine-amino-acid neuropeptide first isolated from rabbit thalamic perfusate in 1977

When dissociated cortical cultures are established from these mice, CSMN are distinguished among many different cortical cells and neurons, as they retain their eGFP expression (Fig