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Schlter A, Aksan B, Fioravanti R, Valente S, Mai A, Mauceri D

Haines MS, Dichtel LE, Santoso K, Torriani M, Miller KK, Bredella MA

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Transfection was performed using 3 l PolyJet (Cat

The following factors were used: 50 ng/mL recombinant EGF, 500 nM A83-01 (Biogems), 100 ng/ mL recombinant Noggin (Peprotech), 10 M Y-27632 (Abmole), 500ng/mL R-spondin-1(Peprotech), 10 mM nicotinamide (Sigma), and 10 M SB202190 (Sigma)