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Iron regulatory protein 1 outcompetes iron regulatory protein 2 in regulating cellular iron homeostasis in response to nitric oxide

The unreacted substrates acted to reduce the Cu(II)neocuproine complex into the strongly coloured Cu(I)neocuproine complex that could be measured spectrophotometrically at 450 nm

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Experiments performed with 5- to 320-L bed volumes packing into a 1mL pipette tip column and agarose-based protein A affinity resin.1 The scalability of PhyTip columns is demonstrated in Figure 3, which shows a clear relationship between the volume of the resin bed and the amount of protein recovered