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Mistake 2: Shaking the vial to dissolve Vigorous shaking creates shearing forces between liquid layers that can damage peptide bonds

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Over time, this can lead to irregular periods, missed ovulation, or your period stopping altogether (called weight loss-related amenorrhea)

The amount of bacteriostatic water you add determines the concentration: For a 5mg vial where you want the entire contents in one injection, adding 0.5 mL of bacteriostatic water gives you 10mg/mL

Inject at a 90-Degree Angle Hold the syringe or pen like a pencil and insert the needle straight into the skin at a 90-degree angle