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Each medication works through distinct pathways: sermorelin stimulates growth hormone release, while tirzepatide activates GLP-1 and GIP receptors to regulate appetite and metabolic function

NHS (diluted 1:200 in veronal buffer) (Complement Technologies), pure human C1q (diluted to 0.366 g/mL in veronal buffer) (Complement Technologies) or pure mouse C1q (diluted to 0.366 g/mL in veronal buffer) (Prospecbio) was added and the plates incubated at 37 C for 30 min