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Soluble A 42 ELISA Hippocampal samples were weighed and homogenized, accordingly, in Tris Buffered Saline EDTA (TBSE) solution (50 mM Tris, 150 mM NaCl, and 2 mM EDTA, pH 7.4) with the addition of 1% protease inhibitor cocktail (1 mL/100 mg tissue, Sigma-Aldrich) using a microtube homogenizer with plastic pestles

The procedure involves the use of medication injected into the tissues, which then destroys the fat cells membranes

: 1

Pseudoinvasion of blood vessels may be present

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