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Viability assay Relative viability was determined in 96 well plates using the CellTiter-Blue Cell Viability Assay (Promega), including a minimum of triplicate wells per sample, vehicle, high concentration vehicle and media only controls

Unlike bacteriostatic water, saline typically contains no preservative, so once a vial or bag is opened, it's intended for single use only, similar to sterile water

Notably, the level of glutamate is relatively high compared with that of GABA, caused by various etiologies, including glutamate release by damage-induced depolarization, exaggerated glutamate release through xc(), and attenuated uptake of glutamate through EAATs, or a decrease in GABA release with subsequent increase in glutamate release

Zhu, G., Dudley, D