Apoptotic populations were identified using a standard Annexin V/PI quadrant gating strategy: Annexin V /PI cells were considered viable cells, Annexin V + /PI cells were classified as early apoptotic cells, Annexin V + /PI + cells as late apoptotic cells, and Annexin V /PI + cells as necrotic cells
Furthermore, HIF-2 represses the transcription of carnitine palmitoyltransferase 1A (CPT1A), which is responsible for mitochondrial -oxidation by transporting fatty acids and results in lipid deposition 78
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To measure catalytic turnover, we used a broader panel of substrates: ABTS, guaiacol, 2,6-dimethoxyphenol (DMP), p -coumaric acid and sinapic acid