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The culture supernatant was harvested by centrifugation at 6000 g for 20 min and clarified by filtration

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The participants were randomized into an aggressive resuscitation group (20 mL/kg rapid bolus followed by 3 mL/kg/h maintenance) and a moderate resuscitation group (hypovolemic patients received a 10 mL/kg bolus followed by 1.5 mL/kg/h maintenance)