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The MTT solution (50 L, 1 mg/mL) was added and incubated for 3 h at 37 C, and then 100 L isopropanol (SigmaAldrich) was used to dissolve the formazan crystals

Heider and Eustis 1994

The expression and activity of cystathionine--lyase and 3-mercaptopyruvate sulfurtransferase in human neoplastic cell lines