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The BRET signal resulting from ligand stimulation was calculated by subtracting the BRET ratio for the vehicle-treated cells from the ligand-stimulated cells of the same aliquot

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References The anti-aging and wound healing properties of copper peptide GHK-Cu

[5] Disruption of these pathways leads to accumulation of homocysteine and methylmalonic acid, metabolic signatures linked to megaloblastic anemia and demyelinating neuropathy

Acute kidney injury (AKI)