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Therefore, you should take the studies above with a grain of salt and always discuss any dietary interventions with your doctor 2) High-Fat Diet A short-term high-fat diet increased TMAO levels during or after meals but did not have long-term effects in 10 non-obese men [48]

The protein and ligand mixtures were incubated on ice for 2 h before vitrification

For detailed reconstitution protocols, see the Reconstitution Guide

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