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However, some contamination may not be visible, which is why following proper storage and handling protocols is critical

To dynamically visualize the calcium activity of small intestine-projecting DMV Ach neurons in freely moving mice, we injected AAV-DIO-GCaMP6m virus into the DMV and AAV2/Retro-hSyn-Cre virus into the small intestine of C57 mice, accompanied with the mounting of a microendoscopic gradient index (GRIN) lens at the top of the DMV (Fig

In this context it should be noted that the kinetics of inhibition of TGR may be rather complex, as discussed above for OPZ

SS: Conceptualization, Methodology, Writing review & editing

Ethics declarations Competing interests WL is cofounder of Refoxy Pharmaceuticals GmbH, Berlin and required by his institution to state so in his publications