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PRIMARY USE:A topical lotion in a water-free base protects the body from cellular damage caused by free radicals

doi: 10.1158/1940-6207.CAPR-19-0258/37877/AM/A-PHASE-I-TRIAL-OF-BERBERINE-IN-CHINESE-WITH 124 RossEAMillerMHPachecoAWillenbergARTigno-AranjuezJTCrawfordKE

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The cells were cultured in synthetic FreeStyle 293 Expression Medium (Thermo Fisher Scientific, #12338018) on the orbital shaker rotating at 135 rpm and placed in the 37 C incubator with a humidified atmosphere of 8% CO 2

Then, it involves other set of the genes ( Braf, Egfr, Egr1, Hdac7, Mapk1, Mapk3, Mapk14, Plcg1, Prkcg, Ptk2, Pxn, Src, Srf, and Vegfa )