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Petrelintide exhibits a potent balanced agonistic effect on both the AMYR and CTRwhich precludes this molecule from being included in the DACRA class [116]

To evaluate the target-binding capacity of D-CuP, molecular docking simulations were conducted using AutoDock Vina against the functional domains of p53 (DNA-binding domain) and HDAC7 (catalytic deacetylase domain)

The protein concentration was determined with the Bradford protein assay (Bio-Rad)

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