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GST protein elution was conducted with 10 mM reduced GSH in 50 mM Tris pH 8.0 or 250 mM imidazole in 20 mM Tris pH 8.0 (300 mM NaCl)

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While certain protein powders may cause flatulence and smelly farts, this does not mean the problem is permanent just because you eat more protein for your dietary needs

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