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Mice were sacrificed at 6, 12, and 24 h post-injection through anesthesia followed by exsanguination via open heart puncture

The technique was developed in 2013, and it involves the use of Cas9 nuclease, which is guided by RNA sequence to the targeted site

BPC-157 is a synthetic 15-amino-acid peptide (stable gastric pentadecapeptide) investigated in research settings for cytoprotective signaling and experimentally reported tissue-repair mechanisms in preclinical models, including pathways relevant to gastrointestinal mucosal integrity, angiogenic signaling, and inflammatory modulation