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After TMB substrate solution is added, only those wells that contain Human CGRP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

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The frozen stock was thawed immediately before use and the reaction was initiated by adding an immobilized enzyme

The article is careful to say these are not routine tests, but describes them as a way to establish whether a real deficit exists before adding a supplement on top of tirzepatide