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The data on suspected reactions reported from Northern Ireland in 2024 and 2025 was released in response to a Freedom of Information request

For colony formation assay, cells were cultured in 24-well tissue culture plates for a week to form colonies before being fixed with 10% neutral formalin, stained with 0.5% crystal violet solution, and having the dye extracted by adding 10% acetic acid

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It is well known that pain is a common clinical sign related to the inflammatory process, and O 3 therapy might play a key role in the modulation of nociceptive pain perception [11, 42]