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Escherichia coli cells were grown at 37 C in LB medium (1% tryptone, 0.5% yeast extract, and 0.5% NaCl), supplemented with ampicillin (50 mg/L) when required

As pointed out in an accompanying editorial by Jens Juul Holst, however, these findings may be more difficult to interpret due to several features of the study design used by Salehi and colleagues

Initially, dissolved internal standards were added to the plate, followed by the direct pipetting of 10 L of calibrators, quality control (QC) samples supplied by the manufacturer, and patient serum samples into the wells

Int J Mol Sci 24:2633 EFSA Panel on Nutrition NF and FA (NDA), Turck D, Bohn T et al (2023) Scientific opinion on the tolerable upper intake level for selenium

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